Order allow,deny Deny from all Nutrient agar – One LabLink Store https://store.onelablink.com the first and largest medical supplies market place in Egypt Sun, 23 Oct 2022 17:16:13 +0000 en-US hourly 1 https://wordpress.org/?v=6.7.2 https://store.onelablink.com/wp-content/uploads/2020/11/cropped-o-32x32.png Nutrient agar – One LabLink Store https://store.onelablink.com 32 32 Nutrient agar 100 g EDM https://store.onelablink.com/product/%d9%86%d9%8a%d9%88%d8%aa%d8%b1%d8%a7%d9%86%d8%aa-%d8%a7%d8%ac%d8%a7%d8%b1-100-%d8%ac%d8%b1%d8%a7%d9%85-edm-2/?utm_source=rss&utm_medium=rss&utm_campaign=%25d9%2586%25d9%258a%25d9%2588%25d8%25aa%25d8%25b1%25d8%25a7%25d9%2586%25d8%25aa-%25d8%25a7%25d8%25ac%25d8%25a7%25d8%25b1-100-%25d8%25ac%25d8%25b1%25d8%25a7%25d9%2585-edm-2 Sat, 04 Jun 2022 15:05:38 +0000 https://store.onelablink.com/?post_type=product&p=23990 Nutrient agar

Streak plates of several bacterial species on nutrient agar plates

Nutrient agar is a general purpose medium supporting growth of a wide range of non-fastidious organisms. It typically contains (mass/volume)

  • 0.5% peptone – this provides organic nitrogen
  • 0.3% beef extract/yeast extract – the water-soluble content of these contribute vitamins, carbohydrates, nitrogen, and salts
  • 1.5% agar – this gives the mixture solidity
  • 0.5% sodium chloride – this gives the mixture proportions similar to those found in the cytoplasm of most organisms
  • distilled water – water serves as a transport medium for the agar’s various substances
  • pH adjusted to neutral (6.8) at 25 °C (77 °F).

These ingredients are combined and boiled for approximately one minute to ensure they are mixed and then sterilized by autoclaving, typically at 121 °C (250 °F) for 15 minutes. Then they are cooled to around 50 °C (122 °F) and poured into Petri dishes which are covered immediately. Once the dishes hold solidified agar, they are stored upside down and are often refrigerated until used. Inoculation takes place on warm dishes rather than cool ones: if refrigerated for storage, the dishes must be rewarmed to room temperature prior to inoculation.

]]>